Immunochemical screening of pesticides (simazine and cypermethrin) in orange oil.

نویسندگان

  • Mikaela Nichkova
  • Xun Fu
  • Zheng Yang
  • Ping Zhong
  • James R Sanborn
  • Dan Chang
  • Shirley J Gee
  • Bruce D Hammock
چکیده

Pesticide residue analysis in citrus oils is very important for their quality and marketing. This study assessed the reliability and sensitivity of enzyme-linked immunosorbent assays (ELISA) for simazine and cypermethrin screening in orange oil. Simazine was analyzed after extraction of the oil with methanolic phosphate buffer with a limit of quantitation (LOQ) of 40 microg/L for 1-fold and approximately 100 microg/L for 10-fold oils. Due to matrix effects the immunoanalysis of cypermethrin required hexane-acetonitrile partitioning followed by silica solid phase extraction. The method detected levels higher than 0.5 ppm (mg/L). This LOQ is lower than the U.S. EPA tolerance level (0.9 ppm) for cypermethrin in citrus oils. A good correlation (r(2) 0.99) between ELISA and LC-MS/MS was observed for the analysis of both analytes in 1-fold orange oil. Immunochemical screening can be used to reduce instrumental analysis costs by its use in preliminary orange oil screening.

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عنوان ژورنال:
  • Journal of agricultural and food chemistry

دوره 57 13  شماره 

صفحات  -

تاریخ انتشار 2009